Services
The services provided (per diem based) include colony maintenance of inbred strains and transgenic lines of rodents utilized in the various research programs as well as providing samples for DNA processing.
Other services include:
- Patient-Derived Xenograft Work
- Tumor Studies
- Necropsy
- Injections
- Surgical Support and Aseptic Training
- Integration with Biological Imaging and Transgenic Mouse Facilities
Zebrafish Services
| Manager | Jennifer Rhodes, PhD [email protected] 215-728-3829 |
| Location & Phone: | Reimann R142 215-728-1559 |
| Pricing & Scheduling: | Contact Jennifer Rhodes for pricing and scheduling. |
| Operating Schedule | Monday to Friday 7:00 am - 9:00 pm weekend and holidays are covered by trained personnel |
The mission of the Zebrafish Facility is to provide support for training, teaching and biomedical research using zebrafish. Fox Chase has established and maintains a program for activities according to the Guide for the Care and Use of Laboratory Animals. The state-of-the-art facility is used to raise and maintain zebrafish, and provides equipment and training to perform microinjections, fin clips, and visual assessment and documentation of embryos. Contact the facility manager to inquire about services, pricing, or to schedule training. All individuals handling zebrafish or the core equipment must be trained by facility staff.
The microinjection equipment can be reserved electronically by qualified individuals.
Mouse Genotyping Services
Mouse Genotyping Services provides cost-effective mouse genotyping (from DNA extraction from mouse tails, toe and ear clips) to support the Laboratory Animal Facility (LAF) and multiple investigators who maintain colonies of transgenic and knockout mice. The data are entered into an on-line mouse genotyping database which tracks animal inventory for each strain of mice.
https://lafbiodb.fccc.edu/login
New assays are developed based on the changing needs of the investigators.
Mouse Genotyping Services occupies approximately 400 square feet on the third floor of the Reinman Building. Laboratory equipment includes an Eppendorf MasterCycler Gradient Thermal Cycler, an MJ Research PTC-225 Thermal Cycler and multiple BioRad T100 Thermal Cyclers. Close access to a QuantStudio6 Pro Real-time PCR System is available.
A Baker SterilGuard II hood is dedicated exclusively to the preparation of PCR reactions. An Eppendorf 5075VAC System is used for automated DNA extractions from tissues and cells. A Qiagen QIAxcel Advanced Instrument is used to visualize PCR products in a capillary electrophoresis format. Refrigerator and freezers (-20°C and -80°C) for reagent and specimen storage and non-circulating water bath are present.