Cell Culture Facility

The Cell Culture Facility provides reagents, equipment, technical expertise, consultation and training to support mammalian cell culture experiments at Fox Chase. Services include:

• Basic cell culture support: cell banking, detection of mycoplasma contamination in cultured cells, cell line validation support, and maintenance of >150 common STR-authenticated cell lines
• On-request establishment of 3D organoid cultures and primary cultures from human and animal tissue samples
• Hybridoma production to generate monoclonal antibodies
• Development of gene-manipulated mouse embryonic stem cells
• Preparation of sterile growth media and reagents
• Specialized equipment: electroporators, cell counter, xCELLigence, low oxygen incubation, Incucyte SX5

  Advantages over other similar shared resources (commercial or academic)

• Discount pricing for standard and custom culture media and reagents
• One-stop source for cell culture expertise, equipment, cell lines, liquid nitrogen cell banking, sterile work bench, and supplies, including media, supplements, and cultureware
• On-site or remote in-person consultation
• Specialized culture media preparation
• Performance-tested fetal bovine serum available at bulk purchase discount
• Only academic shared resource in Philadelphia providing hybridoma production services