The rapid pace of gene discovery over the past decade has significantly expanded efforts to characterize gene function at the molecular, biochemical and cellular levels. High resolution microscopy is an essential tool for characterizing gene and protein functions at the cellular and sub-cellular levels. The Light Microscopy component of Biological Imaging provides users with equipment and training that allows them to monitor cell behavior in real time, and visualize cellular proteins with high spatial and temporal resolution. This facility provides all Cancer Center investigators with a robust suite of imaging technologies, including confocal microscopy, live cell imaging, multiphoton imaging, and image acquisition and processing software for deconvolution microscopy, along with the expertise required to use these tools.
Services
- High-resolution multiplex 3D immunofluorescence
- Live cell imaging of cellular events, cell division and cell motility via short and long time-lapse
- Fluorescence recovery after photobleaching (FRAP)
- Fluorescence Resonance Energy Transfer (FRET)
- Second Harmonic Generation (SHG) imaging
- Clearing and imaging of cleared tissues
- Assisted imaging sessions
- Hands-on training
Additional Services
- Customized image analysis in Fiji, Matlab, Python
- Three-dimensional visualization and analysis in Imaris