Culture Supplies

Media

Common Stocks (Available to all labs all the time)

Below is a list of media preparations available within the facility.  Catalog numbers provide supplier media formulations and any CCF additions are noted.

Special Order (Requires request to be made or permission to obtain)

  • ACL-4 - formulated for primary human kidney tumors and normal kidney
    • Supplement-rich RPMI
  • BME  Sigma B9638  2.2g/L NaHCO3, pH 7.0
  • ES Cell Media
    • DMEM + 15% FBS + HEPES, Pyruvate, L-glutamine, Nucleosides, Non-Essential Amino Acids, L-Arginine, and L-Asparagine.
  • GIST (Gastrointestinal Stromal Tumor)
    • F-10 + 15% FBS + Pen/Strep, L-glutamine, Bovine Pituitary Extract, and Mito+ Serum Extender
  • Grace's Insect Media  Sigma G9771
    • 0.35g/L NaHCO3, pH 5.9
  • High Calcium (1.05mM CaCl2) - formulated for breast tumor lines by the Russo Lab (available on shelf)
    • DMEM/F12 (Special Order) + 5% Horse Serum + EGF, Cholera Toxin, Insulin, Hydrocortisone, and Antibiotic/Antimycotic
  • HOSE (Human Ovarian Surface Epithelial)
    • MCDB-105/Media 199 + 15% FBS + Pen/Strep, L-glutamine, EGF, Insulin, Bovine Pituitary Extract, and Hydrocortisone
  • IIA Complete + 15% FBS
    • Supplement-rich DMEM/F12
    • Excellent for establishing primary fibroblast cell lines
  • Low Calcium (0.04mM CaCl2) - formulated for breast tumor lines by the Russo Lab (available on shelf)
    • DMEM/F12 (Special Order) + 5% CHELATED Horse Serum + EGF, Cholera Toxin, Insulin, Hydrocortisone, and Antibiotic/Antimycotic
    • Excellent for establishing primary epithelial cell lines
  • LP9 Media  M199  Sigma M5017  MCDB 105 Sigma M6395  NaHCO 1.78g/L ,Calf serum,EGF,Hydrocortisone 1mg/ml,Pen/Strep
  • MCDB-105  Sigma M6395
    • No NaHCO3, pH 7.3
  • Media 199  Sigma M5017
    • 2.2g/L NaHCO3, pH 7.0
  • Melanoma Media Tu 2%
    • MCDB-153 (400mL) + L-15 (100mL) + 2% FBS + Insulin and CaCl2
  • Mesothelial Media  RPMI 1640 Sigma R6504 NaHCO 2g/L,FBS,HEPES,ITS,L-Glut,Antibiotics/Antimicotics,Nystatin,alpha ME,Hydrocotisone, Gentamycin
  • Transport Media - antibiotic rich F-12 media for tissue sample transportation
  • Wi-38 Media
    • DMEM + 20% FBS + Pen/Strep, L-glutamine, Non-Essential Amino Acids, Essential Amino Acids, Sodium Pyruvate, and Vitamins

Any specific media requests can be made by filling out our Special Order Media Request Form and emailing the Cell Culture Facility or by dropping the form off in the facility.  Please give up to 3-5 days to complete the request.

Sterile Solutions

  • PBS
  • PBS Ca2+ & Mg2+ Free
  • HBSS
  • HBSS Ca2+ & Mg2+ Free
  • Trypsin
    • 0.04% + EDTA
    • 0.25% + EDTA
    • 0.25% Ca2+ & Mg2+ Free
  • HDF Wash + EDTA
  • 0.1% Swine Skin Gelatin
  • 50mM EDTA Solution
  • Sterile Milli-Q Water
  • DMSO
  • Trypan Blue

Sterile Solutions can be made in 1Liter bottles as per Special Order Media Request Form

Supplements and Supplies

Media Supplements

  • Antibiotic/Antimycotic (100X)
  • Bovine Pituitary Extract 25 mg tube
  • Bovine Serum Albumin (BSA) 1% Solution
  • Calf Serum
  • Charcoal Stripped FBS
  • Cholera Toxin 50µg
  • Ciprofloxacin 10mg/mL (1000X)
  • Dialyzed FBS
  • EDTA 1mM Soln'
  • EGF 10µg/mL
  • Essential Amino Acids (50X)
  • Fetal Bovine Serum (FBS) - Lot tested for optimal growth
  • Fungizone (Amphotericin B) (100X) G418 - 30mg/mL, 50mg/mL Gentamycin Sulfate 10mL & 20ml GlutaMAX (100X)
  • L-Glutamine (100X)
  • Heat Inactivated FBS
  • HEPES (1M) Buffer
  • Horse Serum (also available heat inactivated and charcoal stripped)
  • Hydrocortisone 1mg/ml
  • Hydrocortisone 5mg/ml
  • Hygromycin B 50mg/mL
  • Insulin, Transferrin, Selenium Acid (ITS)
  • Insulin (2500µg/mL)
  • LIF (ESGRO) 50µL
  • Mito+ Serum Extender
  • Non-Essential Amino Acids (NEAA) (100X)
  • Normocin Nystatin (100X)
  • Penicillin/Streptomycin 10,000U/mL//10,000µg/mL (100X)
  • Penicillin, Streptomycin, Kanamycin (PSK) (100X)
  • Puromycin 10mg/mL
  • Sodium Bicarbonate 7.5% w/v
  • T3 (Triiodothyronine) (100X)
  • Transferrin (100X)

Enzymes and Solutions

  • Accutase
  • Collagenase 50mg per 25mL
  • Dispase 10mg/mL (18.3U/mL)
  • 0.04% Trypsin + EDTA
  • 0.25% Trypsin +EDTA
  • 0.25% Trypsin Ca+ & Mg+ free
  • Trypan Blue Stain
  • DMSO 10mL
  • DMSO 100ml

Plasticware

(If you don't see what you need on list, please ask CCF staff)

  • Bottle Top Filters - 150mL and 500mL
  • Cryogenic Vials 2mL
  • Flasks ( T12.5, T25, T75, T182)
  • Freezing Boxes (for long term liquid nitrogen storage) 81 & 100 space
  • Petri Dishes (3.5cm, 6cm, 10cm)
  • Pipets (1, 2, 5, 10, 25, and 50mL)
  • Plates (6, 12, 24, 48, 96 well flat bottom plates)
  • Scalpels
  • Syringe Filters (0.22µm pore size)
  • Tubes (15mL polystyrene, 15mL and 50mL polypropylene)
  • xCELLigence CIM-plate
  • xCELLigence E-plate
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